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ti-e inverted motorised microscope  (Nikon)


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    Structured Review

    Nikon ti-e inverted motorised microscope
    Ti E Inverted Motorised Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti+e+inverted+motorised+microscope/pm39857706-132-6-5
    Average 90 stars, based on 1 article reviews
    ti-e inverted motorised microscope - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microscopy:

    Article Title: Novel Poly-Arginine Peptide R18D Reduces α-Synuclein Aggregation and Uptake of α-Synuclein Seeds in Cortical Neurons
    Article Snippet: Coverslips were mounted onto glass slides using the Fluoromount-GTM mounting medium (ThermoFisher Scientific, R37112). .. Cells were imaged using a Nikon Ti-E inverted motorised microscope with Nikon A1Si spectral detector confocal system using a Plan Apo VC 100× NA1.4 oil immersion objective lens (Nikon, Tokyo, Japan) with a pinhole radius of 40.0 μm. ..

    Article Title: An approach to overcoming regeneration recalcitrance in genetic transformation of lupins and other legumes
    Article Snippet: 1 Centre for Plant Genetics and Breeding (PGB), School of Plant Biology M080, The University of Western Australia, Crawley, WA 6009, Australia 2 School of Plant Biology M090, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia 3 Faculty of Biology, Hanoi University of Science, Hanoi, Vietnam 4 Department of Agriculture and Environment, Centre for Crop and Disease Management, Curtin University, Bentley, WA 6845, Australia 5 Institute of Agriculture M082, The University of Western Australia, Crawley, WA 6009, Australia Received: 25 July 2016 / Accepted: 2 September 2016 © Springer Science+Business Media Dordrecht 2016 An approach to overcoming regeneration recalcitrance in genetic transformation of lupins and other legumes

    Article Title: The Poly-Arginine Peptide R18D Interferes with the Internalisation of α-Synuclein Pre-Formed Fibrils in STC-1 Enteroendocrine Cells
    Article Snippet: Coverslips were mounted onto glass slides using Prolong Diamond Antifade Mountant (#P36970; Invitrogen). .. Slides were imaged using a Nikon Ti-E inverted motorised microscope with Nikon A1Si spectral detector confocal system using a Plan Apo VC 100× NA1.4 oil immersion objective lens (Nikon Corporation, Sydney, Australia) with a 40.0 μm pinhole radius. ..

    Article Title: Using green fluorescent protein sheds light on Lupinus angustifolius L. transgenic shoot development
    Article Snippet: 1 Centre for Plant Genetics and Breeding (PGB), School of Plant Biology M080, The University of Western Australia, Crawley, WA 6009, Australia 2 School of Plant Biology M090, The University of Western Australia, Crawley, WA 6009, Australia 3 Faculty of Biology, Hanoi University of Science, Hanoi, Vietnam 4 Fakultas Pertanian, Universitas Halu Oleo, Kendari, Sulawesi Tenggara, Indonesia 5 Institute of Agriculture M082, The University of Western Australia, Crawley, WA 6009, Australia Received: 30 June 2016 / Accepted: 26 August 2016 © Springer Science+Business Media Dordrecht 2016 Using green fluorescent protein sheds light on Lupinus angustifolius L. transgenic shoot development

    Article Title: Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis.
    Article Snippet: .. Live cell images were acquired on a Nikon A1Si spectral detector confocal system plus TIRF on a Nikon Ti-E inverted motorised microscope with a 60x water objective. .. Cells were incubated in a Tokai Hit stage top incubator (temperature-controlled chamber, 37°C, 5% CO2) in Jo ur na l o f C el l S ci en ce • A cc ep te d m an us cr ip t combination with the Perfect Focus System (PFS) to prevent focus drift.

    Expressing:

    Article Title: An approach to overcoming regeneration recalcitrance in genetic transformation of lupins and other legumes
    Article Snippet: 1 Centre for Plant Genetics and Breeding (PGB), School of Plant Biology M080, The University of Western Australia, Crawley, WA 6009, Australia 2 School of Plant Biology M090, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia 3 Faculty of Biology, Hanoi University of Science, Hanoi, Vietnam 4 Department of Agriculture and Environment, Centre for Crop and Disease Management, Curtin University, Bentley, WA 6845, Australia 5 Institute of Agriculture M082, The University of Western Australia, Crawley, WA 6009, Australia Received: 25 July 2016 / Accepted: 2 September 2016 © Springer Science+Business Media Dordrecht 2016 An approach to overcoming regeneration recalcitrance in genetic transformation of lupins and other legumes

    Article Title: Using green fluorescent protein sheds light on Lupinus angustifolius L. transgenic shoot development
    Article Snippet: 1 Centre for Plant Genetics and Breeding (PGB), School of Plant Biology M080, The University of Western Australia, Crawley, WA 6009, Australia 2 School of Plant Biology M090, The University of Western Australia, Crawley, WA 6009, Australia 3 Faculty of Biology, Hanoi University of Science, Hanoi, Vietnam 4 Fakultas Pertanian, Universitas Halu Oleo, Kendari, Sulawesi Tenggara, Indonesia 5 Institute of Agriculture M082, The University of Western Australia, Crawley, WA 6009, Australia Received: 30 June 2016 / Accepted: 26 August 2016 © Springer Science+Business Media Dordrecht 2016 Using green fluorescent protein sheds light on Lupinus angustifolius L. transgenic shoot development

    Software:

    Article Title: An approach to overcoming regeneration recalcitrance in genetic transformation of lupins and other legumes
    Article Snippet: 1 Centre for Plant Genetics and Breeding (PGB), School of Plant Biology M080, The University of Western Australia, Crawley, WA 6009, Australia 2 School of Plant Biology M090, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia 3 Faculty of Biology, Hanoi University of Science, Hanoi, Vietnam 4 Department of Agriculture and Environment, Centre for Crop and Disease Management, Curtin University, Bentley, WA 6845, Australia 5 Institute of Agriculture M082, The University of Western Australia, Crawley, WA 6009, Australia Received: 25 July 2016 / Accepted: 2 September 2016 © Springer Science+Business Media Dordrecht 2016 An approach to overcoming regeneration recalcitrance in genetic transformation of lupins and other legumes

    Article Title: Using green fluorescent protein sheds light on Lupinus angustifolius L. transgenic shoot development
    Article Snippet: 1 Centre for Plant Genetics and Breeding (PGB), School of Plant Biology M080, The University of Western Australia, Crawley, WA 6009, Australia 2 School of Plant Biology M090, The University of Western Australia, Crawley, WA 6009, Australia 3 Faculty of Biology, Hanoi University of Science, Hanoi, Vietnam 4 Fakultas Pertanian, Universitas Halu Oleo, Kendari, Sulawesi Tenggara, Indonesia 5 Institute of Agriculture M082, The University of Western Australia, Crawley, WA 6009, Australia Received: 30 June 2016 / Accepted: 26 August 2016 © Springer Science+Business Media Dordrecht 2016 Using green fluorescent protein sheds light on Lupinus angustifolius L. transgenic shoot development

    Western Blot:

    Article Title: An approach to overcoming regeneration recalcitrance in genetic transformation of lupins and other legumes
    Article Snippet: 1 Centre for Plant Genetics and Breeding (PGB), School of Plant Biology M080, The University of Western Australia, Crawley, WA 6009, Australia 2 School of Plant Biology M090, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia 3 Faculty of Biology, Hanoi University of Science, Hanoi, Vietnam 4 Department of Agriculture and Environment, Centre for Crop and Disease Management, Curtin University, Bentley, WA 6845, Australia 5 Institute of Agriculture M082, The University of Western Australia, Crawley, WA 6009, Australia Received: 25 July 2016 / Accepted: 2 September 2016 © Springer Science+Business Media Dordrecht 2016 An approach to overcoming regeneration recalcitrance in genetic transformation of lupins and other legumes

    Article Title: Using green fluorescent protein sheds light on Lupinus angustifolius L. transgenic shoot development
    Article Snippet: 1 Centre for Plant Genetics and Breeding (PGB), School of Plant Biology M080, The University of Western Australia, Crawley, WA 6009, Australia 2 School of Plant Biology M090, The University of Western Australia, Crawley, WA 6009, Australia 3 Faculty of Biology, Hanoi University of Science, Hanoi, Vietnam 4 Fakultas Pertanian, Universitas Halu Oleo, Kendari, Sulawesi Tenggara, Indonesia 5 Institute of Agriculture M082, The University of Western Australia, Crawley, WA 6009, Australia Received: 30 June 2016 / Accepted: 26 August 2016 © Springer Science+Business Media Dordrecht 2016 Using green fluorescent protein sheds light on Lupinus angustifolius L. transgenic shoot development



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    Fig. 2. The P2X7 receptor (P2X7R) channel is functional in human glioblastoma cancer stem cells (GSCs). GSCs at 80 % confluency were treated with Fluo-4 AM, a synthetic fluorescent indicator, for 30 minutes. To confirm a P2X7R-specific response, some cells were also treated for 15 minutes with 25μM of the specific P2X7R antagonist, AZ10606120 (AZ). Cells were immersed in Dulbecco’s PBS and imaged live with a Nikon A1r <t>fluorescence</t> confocal <t>microscope</t> at 40× magnification over an 8-minute time-series experiment. BzATP (200μM), a P2X7R agonist, was added at approximately 40 seconds in. P2X7R channel activity was determined as the relative fold change in fluorescence intensity (FI) from the baseline after the addition of bzATP (ΔFI/FIBaseline). A) BzATP stimulation induced P2X7R channel responses. There was a significant decrease in Fluo-4 FI in GSCs treated with AZ. Results were yielded from an unpaired t-test with Welch’s correction; n=12 replicates, t(21.23)=8.569, mean difference=-4.12, 95 % CI=-5.114 to −3.118, ****p<0.0001. Data are presented as mean±SEM. B) Graphical representation of the change in FI across 8 minutes in cells treated with Fluo-4 and cells treated with Fluo-4 + AZ. C) Representative timeseries images of FI across 8 minutes in a sample exposed to Fluo-4. D) Representative timeseries images of FI across 8 minutes in a sample exposed to Fluo-4 and pre-treated with AZ.
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    Fig. 2. The P2X7 receptor (P2X7R) channel is functional in human glioblastoma cancer stem cells (GSCs). GSCs at 80 % confluency were treated with Fluo-4 AM, a synthetic fluorescent indicator, for 30 minutes. To confirm a P2X7R-specific response, some cells were also treated for 15 minutes with 25μM of the specific P2X7R antagonist, AZ10606120 (AZ). Cells were immersed in Dulbecco’s PBS and imaged live with a Nikon A1r <t>fluorescence</t> confocal <t>microscope</t> at 40× magnification over an 8-minute time-series experiment. BzATP (200μM), a P2X7R agonist, was added at approximately 40 seconds in. P2X7R channel activity was determined as the relative fold change in fluorescence intensity (FI) from the baseline after the addition of bzATP (ΔFI/FIBaseline). A) BzATP stimulation induced P2X7R channel responses. There was a significant decrease in Fluo-4 FI in GSCs treated with AZ. Results were yielded from an unpaired t-test with Welch’s correction; n=12 replicates, t(21.23)=8.569, mean difference=-4.12, 95 % CI=-5.114 to −3.118, ****p<0.0001. Data are presented as mean±SEM. B) Graphical representation of the change in FI across 8 minutes in cells treated with Fluo-4 and cells treated with Fluo-4 + AZ. C) Representative timeseries images of FI across 8 minutes in a sample exposed to Fluo-4. D) Representative timeseries images of FI across 8 minutes in a sample exposed to Fluo-4 and pre-treated with AZ.
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    Fig. 2. The P2X7 receptor (P2X7R) channel is functional in human glioblastoma cancer stem cells (GSCs). GSCs at 80 % confluency were treated with Fluo-4 AM, a synthetic fluorescent indicator, for 30 minutes. To confirm a P2X7R-specific response, some cells were also treated for 15 minutes with 25μM of the specific P2X7R antagonist, AZ10606120 (AZ). Cells were immersed in Dulbecco’s PBS and imaged live with a Nikon A1r <t>fluorescence</t> confocal <t>microscope</t> at 40× magnification over an 8-minute time-series experiment. BzATP (200μM), a P2X7R agonist, was added at approximately 40 seconds in. P2X7R channel activity was determined as the relative fold change in fluorescence intensity (FI) from the baseline after the addition of bzATP (ΔFI/FIBaseline). A) BzATP stimulation induced P2X7R channel responses. There was a significant decrease in Fluo-4 FI in GSCs treated with AZ. Results were yielded from an unpaired t-test with Welch’s correction; n=12 replicates, t(21.23)=8.569, mean difference=-4.12, 95 % CI=-5.114 to −3.118, ****p<0.0001. Data are presented as mean±SEM. B) Graphical representation of the change in FI across 8 minutes in cells treated with Fluo-4 and cells treated with Fluo-4 + AZ. C) Representative timeseries images of FI across 8 minutes in a sample exposed to Fluo-4. D) Representative timeseries images of FI across 8 minutes in a sample exposed to Fluo-4 and pre-treated with AZ.
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    Image Search Results


    Fig. 2. The P2X7 receptor (P2X7R) channel is functional in human glioblastoma cancer stem cells (GSCs). GSCs at 80 % confluency were treated with Fluo-4 AM, a synthetic fluorescent indicator, for 30 minutes. To confirm a P2X7R-specific response, some cells were also treated for 15 minutes with 25μM of the specific P2X7R antagonist, AZ10606120 (AZ). Cells were immersed in Dulbecco’s PBS and imaged live with a Nikon A1r fluorescence confocal microscope at 40× magnification over an 8-minute time-series experiment. BzATP (200μM), a P2X7R agonist, was added at approximately 40 seconds in. P2X7R channel activity was determined as the relative fold change in fluorescence intensity (FI) from the baseline after the addition of bzATP (ΔFI/FIBaseline). A) BzATP stimulation induced P2X7R channel responses. There was a significant decrease in Fluo-4 FI in GSCs treated with AZ. Results were yielded from an unpaired t-test with Welch’s correction; n=12 replicates, t(21.23)=8.569, mean difference=-4.12, 95 % CI=-5.114 to −3.118, ****p<0.0001. Data are presented as mean±SEM. B) Graphical representation of the change in FI across 8 minutes in cells treated with Fluo-4 and cells treated with Fluo-4 + AZ. C) Representative timeseries images of FI across 8 minutes in a sample exposed to Fluo-4. D) Representative timeseries images of FI across 8 minutes in a sample exposed to Fluo-4 and pre-treated with AZ.

    Journal: Brain research bulletin

    Article Title: P2X7 receptor antagonism by AZ10606120 significantly depletes glioblastoma cancer stem cells in vitro.

    doi: 10.1016/j.brainresbull.2024.110996

    Figure Lengend Snippet: Fig. 2. The P2X7 receptor (P2X7R) channel is functional in human glioblastoma cancer stem cells (GSCs). GSCs at 80 % confluency were treated with Fluo-4 AM, a synthetic fluorescent indicator, for 30 minutes. To confirm a P2X7R-specific response, some cells were also treated for 15 minutes with 25μM of the specific P2X7R antagonist, AZ10606120 (AZ). Cells were immersed in Dulbecco’s PBS and imaged live with a Nikon A1r fluorescence confocal microscope at 40× magnification over an 8-minute time-series experiment. BzATP (200μM), a P2X7R agonist, was added at approximately 40 seconds in. P2X7R channel activity was determined as the relative fold change in fluorescence intensity (FI) from the baseline after the addition of bzATP (ΔFI/FIBaseline). A) BzATP stimulation induced P2X7R channel responses. There was a significant decrease in Fluo-4 FI in GSCs treated with AZ. Results were yielded from an unpaired t-test with Welch’s correction; n=12 replicates, t(21.23)=8.569, mean difference=-4.12, 95 % CI=-5.114 to −3.118, ****p<0.0001. Data are presented as mean±SEM. B) Graphical representation of the change in FI across 8 minutes in cells treated with Fluo-4 and cells treated with Fluo-4 + AZ. C) Representative timeseries images of FI across 8 minutes in a sample exposed to Fluo-4. D) Representative timeseries images of FI across 8 minutes in a sample exposed to Fluo-4 and pre-treated with AZ.

    Article Snippet: Cells were imaged with the Nikon Ti-E inverted motorised fluorescence microscope at 20× objective.

    Techniques: Functional Assay, Fluorescence, Microscopy, Activity Assay

    Fig. 3. The P2X7 receptor (P2X7R) pore is functional in human glioblas toma cancer stem cells (GSCs). GSCs at 80 % confluency were exposed to YOPRO-1 Iodide (YP), a large nucleic acid stain permeant to cells with P2X7R pore activation, for 30 minutes across three treatment groups: YP only; YP and bzATP (P2X7R agonist, 200μM); and YP, bzATP and AZ10606120 (P2X7R antagonist, 1μM, 15 minutes). Cells were immersed in PBS and imaged live with a Nikon Ti-E fluorescence microscope at 40× magnification. Mean YP fluores cence intensity (FI) was quantified for each treatment group. A) There was a significant decrease in YP FI in GSCs treated with AZ. Results were yielded from a one-way ANOVA with Dunnett’s T3 multiple comparisons test, F(2, 51.84)= 14.04, p<0.0001. N=23 replicates. YP versus YP + bzATP mean difference=- 241.5, 95 % CI=-483.1–0.03, p=0.05; YP versus YP + bzATP + AZ mean dif ference=206.7, 95 % CI=21.54–391.8, *p=0.03; YP + bzATP versus YP + bzATP + AZ mean difference=448.2, 95 % CI=251.4–645, ****p<0.0001. Data are presented as mean±SEM. B) Image representation of YP FI for each treat ment group.

    Journal: Brain research bulletin

    Article Title: P2X7 receptor antagonism by AZ10606120 significantly depletes glioblastoma cancer stem cells in vitro.

    doi: 10.1016/j.brainresbull.2024.110996

    Figure Lengend Snippet: Fig. 3. The P2X7 receptor (P2X7R) pore is functional in human glioblas toma cancer stem cells (GSCs). GSCs at 80 % confluency were exposed to YOPRO-1 Iodide (YP), a large nucleic acid stain permeant to cells with P2X7R pore activation, for 30 minutes across three treatment groups: YP only; YP and bzATP (P2X7R agonist, 200μM); and YP, bzATP and AZ10606120 (P2X7R antagonist, 1μM, 15 minutes). Cells were immersed in PBS and imaged live with a Nikon Ti-E fluorescence microscope at 40× magnification. Mean YP fluores cence intensity (FI) was quantified for each treatment group. A) There was a significant decrease in YP FI in GSCs treated with AZ. Results were yielded from a one-way ANOVA with Dunnett’s T3 multiple comparisons test, F(2, 51.84)= 14.04, p<0.0001. N=23 replicates. YP versus YP + bzATP mean difference=- 241.5, 95 % CI=-483.1–0.03, p=0.05; YP versus YP + bzATP + AZ mean dif ference=206.7, 95 % CI=21.54–391.8, *p=0.03; YP + bzATP versus YP + bzATP + AZ mean difference=448.2, 95 % CI=251.4–645, ****p<0.0001. Data are presented as mean±SEM. B) Image representation of YP FI for each treat ment group.

    Article Snippet: Cells were imaged with the Nikon Ti-E inverted motorised fluorescence microscope at 20× objective.

    Techniques: Functional Assay, Staining, Activation Assay, Fluorescence, Microscopy

    Fig. 4. P2X7 receptor (P2X7R) antagonism by AZ10606120 (AZ) and its comparison with conventional temozolomide (TMZ) chemotherapy in human glioblastoma stem cells (GSCs). GSCs were treated at 80 % confluency with 5, 15, 25, 50 or 100μM of AZ, or 50μM of TMZ for 72 hours. The control group were untreated cells. Cells were fixed and stained with DAPI. Images were acquired with a Nikon Ti-E inverted fluorescence motorised microscope at 20× objective. The total number of DAPI+ cells were quantified across 16 random fields for each sample and treatment group. A) There was significant GSC depletion with AZ con centrations of 15μM or greater. Results were yielded from a one-way ANOVA with Dunnett’s T3 post-hoc, F(5, 46.73)=58.35, p<0.0001. N=12 replicates. Untreated versus AZ 5μM: mean difference=1232 cells, 95 % CI=-669.1–3134, p=0.67; Untreated versus AZ 15μM: mean difference=4248 cells 95 % CI=2425–6071; Un treated versus AZ 25μM: mean difference=5150 cells, 95 % CI= 3391–6910; Untreated versus AZ 50μM: mean difference=7065 cells, 95 % CI=5630–8500; Un treated versus AZ 100μM: mean difference=7006 cells, 95 % CI= 5583–8428. ****p<0.0001. B) Comparison of 15μM AZ with TMZ in GSCs. Results were yielded from a one-way ANOVA with Dunnett’s T3 post-hoc, F(3, 95.36)=18.84, p<0.0001. N=32 replicates. Untreated versus AZ 15μM: mean difference=4590 cells, 95 % CI=2215–6964; Untreated versus TMZ: mean difference=2002 cells, 95 % CI=-298.9–4303, p=0.12; Untreated versus AZ+TMZ: mean difference=4645 cells, 95 % CI=2556–6734; AZ 15μM versus TMZ: mean difference=-2587 cells, 95 % CI=-4494 to −680.6; AZ 15μM versus AZ+TMZ: mean difference=55.47 cells, 95 % CI=- 1576–1687, p>0.999; TMZ versus AZ+TMZ: mean difference=2643 cells, 95 % CI=1132–4154 **p=0.003, ****p<0.0001. Data are presented as mean±SEM. C) Image representation of GSC monolayers treated with 15μM AZ, TMZ, AZ+TMZ and control.

    Journal: Brain research bulletin

    Article Title: P2X7 receptor antagonism by AZ10606120 significantly depletes glioblastoma cancer stem cells in vitro.

    doi: 10.1016/j.brainresbull.2024.110996

    Figure Lengend Snippet: Fig. 4. P2X7 receptor (P2X7R) antagonism by AZ10606120 (AZ) and its comparison with conventional temozolomide (TMZ) chemotherapy in human glioblastoma stem cells (GSCs). GSCs were treated at 80 % confluency with 5, 15, 25, 50 or 100μM of AZ, or 50μM of TMZ for 72 hours. The control group were untreated cells. Cells were fixed and stained with DAPI. Images were acquired with a Nikon Ti-E inverted fluorescence motorised microscope at 20× objective. The total number of DAPI+ cells were quantified across 16 random fields for each sample and treatment group. A) There was significant GSC depletion with AZ con centrations of 15μM or greater. Results were yielded from a one-way ANOVA with Dunnett’s T3 post-hoc, F(5, 46.73)=58.35, p<0.0001. N=12 replicates. Untreated versus AZ 5μM: mean difference=1232 cells, 95 % CI=-669.1–3134, p=0.67; Untreated versus AZ 15μM: mean difference=4248 cells 95 % CI=2425–6071; Un treated versus AZ 25μM: mean difference=5150 cells, 95 % CI= 3391–6910; Untreated versus AZ 50μM: mean difference=7065 cells, 95 % CI=5630–8500; Un treated versus AZ 100μM: mean difference=7006 cells, 95 % CI= 5583–8428. ****p<0.0001. B) Comparison of 15μM AZ with TMZ in GSCs. Results were yielded from a one-way ANOVA with Dunnett’s T3 post-hoc, F(3, 95.36)=18.84, p<0.0001. N=32 replicates. Untreated versus AZ 15μM: mean difference=4590 cells, 95 % CI=2215–6964; Untreated versus TMZ: mean difference=2002 cells, 95 % CI=-298.9–4303, p=0.12; Untreated versus AZ+TMZ: mean difference=4645 cells, 95 % CI=2556–6734; AZ 15μM versus TMZ: mean difference=-2587 cells, 95 % CI=-4494 to −680.6; AZ 15μM versus AZ+TMZ: mean difference=55.47 cells, 95 % CI=- 1576–1687, p>0.999; TMZ versus AZ+TMZ: mean difference=2643 cells, 95 % CI=1132–4154 **p=0.003, ****p<0.0001. Data are presented as mean±SEM. C) Image representation of GSC monolayers treated with 15μM AZ, TMZ, AZ+TMZ and control.

    Article Snippet: Cells were imaged with the Nikon Ti-E inverted motorised fluorescence microscope at 20× objective.

    Techniques: Comparison, Control, Staining, Fluorescence, Microscopy

    Fig. 7. Cleaved caspase-3 staining in human glioblastoma cancer stem cells (GSCs) following P2X7 receptor (P2X7R) inhibition by AZ10606120 (AZ). GSCs at 80 % confluency were fixed and stained with primary rabbit anti-cleaved caspase-3 antibody and secondary goat anti-rabbit Alexa Fluor 488. Cell nuclei were subsequently stained with DAPI. Cells were imaged with a Nikon Ti-E fluorescence microscope at 20× magnification. Mean cleaved caspase-3 fluorescence intensity (FI) was quantified for each treatment group across an average of at least 100 cells. A) There was no significant difference in cleaved caspase-3 FI between GSCs treated with AZ and untreated GSCs: t(5.25)=0.22, mean difference=-12.88, 95 % CI=-164.7–138.9, p=0.84. Results were yielded from an unpaired t-test with Welch’s correction. N=6 replicates. Data are presented as mean±SEM. B) Image representation of cleaved caspase-3 FI in untreated GSCs. C) Image representation of cleaved caspase-3 FI in GSCs treated with AZ 15μM.

    Journal: Brain research bulletin

    Article Title: P2X7 receptor antagonism by AZ10606120 significantly depletes glioblastoma cancer stem cells in vitro.

    doi: 10.1016/j.brainresbull.2024.110996

    Figure Lengend Snippet: Fig. 7. Cleaved caspase-3 staining in human glioblastoma cancer stem cells (GSCs) following P2X7 receptor (P2X7R) inhibition by AZ10606120 (AZ). GSCs at 80 % confluency were fixed and stained with primary rabbit anti-cleaved caspase-3 antibody and secondary goat anti-rabbit Alexa Fluor 488. Cell nuclei were subsequently stained with DAPI. Cells were imaged with a Nikon Ti-E fluorescence microscope at 20× magnification. Mean cleaved caspase-3 fluorescence intensity (FI) was quantified for each treatment group across an average of at least 100 cells. A) There was no significant difference in cleaved caspase-3 FI between GSCs treated with AZ and untreated GSCs: t(5.25)=0.22, mean difference=-12.88, 95 % CI=-164.7–138.9, p=0.84. Results were yielded from an unpaired t-test with Welch’s correction. N=6 replicates. Data are presented as mean±SEM. B) Image representation of cleaved caspase-3 FI in untreated GSCs. C) Image representation of cleaved caspase-3 FI in GSCs treated with AZ 15μM.

    Article Snippet: Cells were imaged with the Nikon Ti-E inverted motorised fluorescence microscope at 20× objective.

    Techniques: Staining, Inhibition, Fluorescence, Microscopy